run("Set Scale...", "distance=1 known=5.3858 unit=um");
//run("Brightness/Contrast...");
run("Enhance Contrast", "saturated=0.35");
run("Apply LUT", "stack");
run("Close");
//setTool("oval");
makeOval(54, 80, 770, 778);
makeOval(2, 10, 770, 778);
makeOval(2, 10, 854, 778);
makeOval(2, 10, 854, 844);
makeOval(2, 2, 854, 852);
run("Crop");
//setTool("rectangle");
//setTool("hand");
makeOval(0, 0, 854, 852);
setAutoThreshold("Default dark");
//run("Threshold...");
run("Close");
setBackgroundColor(0, 0, 0);
run("Clear Outside", "stack");
//run("Threshold...");
setAutoThreshold("Otsu dark");
saveAs("Tiff", "C:/Users/......./Doddington_Sandstone/dodd_5.3858_Cropped.tif");
setOption("BlackBackground", true);
run("Convert to Mask", "method=Otsu background=Dark calculate black");
run("Close");