FastQCFastQC Report
Tue 21 May 2013
Sample_Yeast_L005_R1.cat.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename Sample_Yeast_L005_R1.cat.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 592180
Filtered Sequences 0
Sequence length 101
%GC 40

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGC 2803 0.4733358100577527 TruSeq Adapter, Index 1 (97% over 37bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 110810 4.19239 7.96175 15-19
TTTTT 352785 3.5414593 5.207852 10-14
GAAGA 138530 1.7625622 6.7152853 6
GGAAG 87000 1.5917709 8.4951315 5
AAGAG 113955 1.4498866 6.222126 7
GAGCA 63940 1.2896557 8.680826 9
AGAGC 63350 1.2777555 8.778584 8
CGGAA 47700 0.96209836 8.886041 4
ATCGG 33030 0.7347307 9.608717 2
TCGGA 32035 0.7125976 9.315497 3
GATCG 28845 0.64163816 8.56018 1
Produced by FastQC (version 0.10.1)