FastQCFastQC Report
Wed 5 Jul 2017
5knuclei_S77_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5knuclei_S77_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36947278
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG151750.017.6894444
GTCCTAC212750.014.8827031
GTAGGAC229550.014.01407053
GTCCTAA186400.013.69008351
TACACTG275200.013.5987495
AGGACGT198000.013.412365
GTATAGG113750.012.2654421
GGACGTG198700.012.2408726
TACAGTG326700.012.1159545
GTATAGT145450.011.9281971
TGTAGGA352800.011.8925252
CCGTACG39150.011.7638949
GTCCTAG248750.011.7116821
CTATACT203550.011.632124
CTACACT244050.011.5300724
TACACCG56200.011.5252485
TCCTACA359100.011.5035152
TTAGGAC197100.011.4285673
GTCTAGA219500.011.3621851
TATACTG161600.011.3564595
Produced by FastQC (version 0.11.3)