FastQCFastQC Report
Wed 5 Jul 2017
5knuclei_S77_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5knuclei_S77_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36947278
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT598200.16190637913840364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG154050.018.3956434
GTAGGAC238950.015.7833613
GTCCTAC220350.015.6604991
CGTCTAG54500.014.782751
GTATAGT144550.014.4518291
AGGACGT203950.014.4170075
GTGTAGG264900.013.7534691
GTATAGG109500.013.608351
GGACGTG200150.013.5904936
TACACTG274650.013.5244815
CTATACT185150.013.4644064
TGTAGGA366900.013.086372
GTCCTAG244800.012.7275071
GTCCTAA187000.012.3531211
CTAGACT216700.012.2579124
GTGTAGA215900.012.2186261
TAGACTG243700.012.2111625
ATACGCG38550.012.1597144
TACAGTG339650.012.1072485
TAGGACA227700.011.977664
Produced by FastQC (version 0.11.3)