FastQCFastQC Report
Wed 5 Jul 2017
50knuclei_S56_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename50knuclei_S56_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10238063
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC5205.8405985E-613.86234951
TAGGACG43300.013.8007264
GTCCTAC61450.013.255521
TACACCG12950.012.2309565
TATACTG65950.012.2267425
TACACAG149000.011.6932875
GTAGGAC64800.011.666113
GTCTAGG55600.011.5386611
TACACTG111000.011.4155595
AGGACCG29650.011.1696785
AGGACGT52400.011.1291315
CTAGGAC69950.010.9101293
CTATACT61650.010.7440124
CTACACT78750.010.6966314
GTGTAGG73300.010.6208681
CTACACA138200.010.5754724
CCTACAC75750.010.5499923
TATACAG89400.010.5497285
TCTACAC80050.010.4329813
ACTGTGC105700.010.421358
Produced by FastQC (version 0.11.3)